We are again proud to present an excellent volume of contemporary topics in NMR and EPR to the biological community. The philosophy behind the volume and the presentation of each chapter remains at the high level reflected in our earlier volumes: to be current, pedagogical, and critical. The first chapters, as always, address a subject related to in-vivo biology. Gabby Elgavish addresses NMR spectroscopy of the intact heart. lain Campbell and colleagues present a state-of-the-art description of NMR methods for probing enzyme kinetics in intact cells and tissues. Klaus Mobius and Wolfgang Lubitz have produced a thorough review of the principles and applications of ENDOR spectroscopy in photobiology and biochemistry including discussions of liquid and solid state ENDOR as well as CIDEP-enhanced ENDOR. The final chapter by Hans Vogel and Sture Forsen addresses a contemporary problem in inorganic biochemistry, namely cation binding to calcium binding proteins. We are pleased to announce that a special forthcoming volume will be devoted entirely to the subject of "Spin Labeling: Theory and Applications (3rd compendium)." A substantial degree of progress has occurred in this important area of ESR in biology since the last treatise on the subject in 1979. Lastly, we acknowledge our colleagues in the field who continue to support this excellent series both as subscribers and contributors. We pledge to continue servicing the community as long as the need exists.
We are pleased to present Volume 9 of our highly successful series, which now celebrates 12 years of providing the magnetic resonance community with topical, authoritative chapters on new aspects of biological magnetic resonance. As always, we try to present a diversity of topic coverage in each volume, ranging from applications of in vivo magnetic resonance to more fundamental aspects of electron spin resonance and nuclear magnetic resonance. Philip Yeagle presents an eagerly awaited chapter on 31p NMR studies of membranes and membrane protein interactions. Alan Marshall has con tributed two chapters to the volume: one, with Jiejun Wu, describes magnetic resonance studies of 5S-RNA as probes of its structure and conformation; the secon
We take great pleasure in presenting Vol. IV of Biological Magnetic Resonance, a series that continues to give us pride. In this volume, we are pleased to have our first chapter on the applications of ESR to problems in medicine, written by Butterfield. Armitage and Otvos describe their extensive Cd-l13 NMR study in a chapter that should delight the spectroscopists as well as the biochemists, since the systems investigated have not yet been modeled. Kaptein presents an eloquent exposition of the principles and applications to biological systems of the photo-CIDNP technique, to which he has made pioneering contributions. Perkins tells everything one always wanted to know about the applications of ring current calculations in structural studies of biological macromolecules. Our philosophy has been, and continues to be, to present topics of current interest by authors who are active in their field, while maintaining the inter national flavor of the series. Ideally, the coverage of each topic should approach that found both in a textbook and in a reference book, rather than being a mere literature review. We are grateful to the authors for their cooperation in this respect. We continue to solicit the comments and suggestions of our readers and our colleagues, and thank those who have already responded, including the reviewers in the periodicals. Lawrence J. Berliner Jacques Reuben ix Contents Chapter 1 Spin Labeling in Disease D. Allan Butterfield 1. Introduction ...................................... . 2. Membrane Structure . . . . . . . . . . . . . . . . . . . . . . . . . . 2 . . . . . . . 2.1. General Principles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2.2. The Erythrocyte Membrane. . . . . . . . . . . . . . . . . . . 4 . . . . .
We have now reached our sixth volume in a series which has somewhat unintentionally become an annual event. While we still intend to produce a volume only if a suitable number of excellent chapters in the forefront of biological magnetic resonance are available, our philosophy is to present a pedagogical yet critical description and review of selected topics in mag netic resonance of current interest to the community of biomedical scien tists. This volume fulfills our goals well. As always, we open the volume with a chapter which directly addresses an in vivo biological problem: Phil Bolton's presentation of new techniques in measuring 31 P NMR in cells. Lenkinski's chapter on the theory and applications of lanthanides in protein studies covers the details, highlights, and pitfalls of analysis of these com plexes in biochemical NMR. Reed and Markham summarize the interpreta tion of EPR spectra of manganese in terms of structure and function of proteins and enzymes. Dalton and colleagues describe the applications to biological problems of the relatively new capability of time domain ESR. Finally, we are pleased to offer a departure from mainstream magnetic resonance with the comprehensive and stimulating chapter by Gus Maki on the theory, instrumentation, and applications of optically detected magnetic resonance.
We are pleased to present this second volume of a series that has already received much interest. The application of magnetic resonance methods to the study of actual biological systems as contrasted to cell-free samples, although not entirely novel, as demonstrated by Civan and Shporer in Volume I, has taken on new dimensions with the use of phosphorus-31 and carbon-13 NMR in studying cells, tissues, and organelles. The applications of 31 P NMR to such systems is reviewed in this volume, while carbon-13 will be covered in a later one. The use of nitroxide spin labels has grown to the point where it now may be considered a common biological technique. The synthesis and applications of a new class of nitroxides is described in this volume. ESR spectroscopy of paramagnetic ions is a powerful approach to studying molecular and structural details, as the chapter by Boas, Pilbrow, and Smith on the ESR of copper in Volume 1 has shown. In this volume the ESR of molybdenum and iron is treated in a comparable fashion. In the first volume some aspects of 1 H NMR spectroscopy of certain classes of In this volume the high-resolu biological macromolecules were discussed.· tion multinuclear NMR spectra of peptides, including the physiologically significant peptide hormones, are reviewed.
Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features: Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation
We are proud to present Volume 3 of Biological Magnetic Resonance, a series that has met with praise from the scientific community. This volume covers the new applications of various multiple irradia tion techniques to the NMR of biomolecules; the chapter of Keller and Wuthrich describes much of the technique and its applications to hemo proteins. The ESR of some hemoproteins in the single crystal is described by Chien and Dickinson, who also include discussions of techniques and methods for single-crystal ESR of paramagnetically intrinsic and spin labeled protein crystals. Mims and Peisach describe the latest applications and results in electron spin echo spectroscopy of several metalloproteins. Two ESR spin probe techniques are reviewed. Chasteen describes the methods and applications of vanadyl(JV) to several systems. Ohnishi and Tokutomi describe studies of phase separations in mixed and model mem branes by the nitroxide spin probe technique. We have been successful in continuing to provide topics that are timely and experimentally informative with a heavy emphasis on biolo gically relevant applications. We thank our colleagues in the scientific com munity for their suggestions on future coverage-we will remain receptive to future suggestions and comments on this series. A tentative topic list for forthcoming volumes is given on the following pages.
Judging from the articles published in Biochemistry, magnetic resonance techniques (NMR and ESR) are now among the most popular methods in biochemical research. The series Biological Magnetic Resonance, the fifth volume of which we are proudly presenting, is intended to provide authori tative coverage of topics of current interest. Previous volumes have covered a number of aspects in a thorough and pedagogical fashion rarely found in other publications in this field. Continuing to fulfill the mission of the series, this volume presents a chapter by Baxter, Mackenzie, and Scott on the applications of carbon-13 NMR spectroscopy in investigations of methabolic pathways in vivo. Blom berg and Ruterjans give a comprehensive summary of the use of nitrogen-15 NMR in studies of systems of biological interest. Phosphorus-3I NMR investigations of enzyme systems are described by Rao. Tsai and Bruzik outline the principles of and summarize the state-of-the-art advances in the 18 use of oxygen isotopes e 70 and 0) in phosphorus-3I and oxygen-17 NMR studies of biophosphates. Lipid-protein interactions as reflected in ESR and NMR data are discussed by Devaux. We wish to thank the authors for their cooperation in maintaining the and continued high standards of the series.
This volume constitutes a compilation of the latest experiments and theories on a rapidly evolving and maturing field in MRI/MRS, which is the use of the stable isotope 13-C. The 13-C is used to probe the chemistry, mechanism, and function in living systems. All the chapters are written by experts in the field who discuss topics such as `Tracer Theory and the Suitability of 13-C NMR', `Applications of 13-C to Studies of Human Brain Metabolism', etc.