The first edition of this book covered the basic treatment of the enzyme reaction using the overall reaction kinetics and stopped-flow method, the general properties of protein and cofactors, the control of enzyme reaction, and the preparation of enzyme protein. These topics are the basis of enzyme research and thus suitable for the beginner in the field. The second edition presents the cofactors produced via the post-translational modification of the enzyme’s active site. These cofactors expand the function of enzymes and open a new research field. The carbonyl reagent phenylhydrazine and related compounds have been useful in finding some of the newly discovered cofactors and thus have been discussed in this edition. The topic of the control of enzyme activity through the channel of substrates and products in polyfunctional enzymes has also been expanded in this book.
For a long time, enzymes have been studied by measuring their activity, which has led to the advancement of "enzyme kinetics." In recent years, the mechanism of enzyme reaction has been explained in detail on the basis of the 3D structure. Genetic engineering and the 3D structural analysis of enzymes contribute to these advancements in enzymology. This book starts with an introduction to various enzymes to show how interesting enzymes are, which is followed by historical kinetic studies on enzymes and the overall and rapid-reaction kinetics. The subsequent topics describe the basics of protein structure, the control of enzyme activity, and the purification of enzymes. A case on the kinetic and structural studies of l-phenylalanine oxidase is also presented. There are many good books on enzyme kinetics, but few describe their kinetic and structural aspects. This book deals with both and contains many references that can be good sources for further reading. It is handy and is especially helpful for beginners. A number of figures, including some with stereo expression, facilitate observing the 3D structure of enzymes.
Enzymes are the critical ingredient in every organism that make life possible. They are catalysts and drive every chemical reaction that takes place in the human body, enabling our bodies to be built from proteins, carbohydrates, and fats. In this program we investigate the role of enzymes in detail, offering both theoretical and practical examples. We look at how enzymes function in living organisms, enzyme kinetics, enzyme substrate complex, enzyme activity, and finally the power of enzymes in the 21st century. We demonstrate enzyme activity, and the affects of substrate concentration, the pH, temperature and inhibitors.
Eagan Press is the food science publishing imprint of AACC. The goal of the Eagan Press Ingredient Handbook Series is to create a single source of practical information for each of the major ingredients used in food processing. These handbooks fill the gap between scientific literature and the product specific information provided by suppliers. The result is a series of books that help food industry professionals gain a common understanding of ingredients, their properties, and their applications. Puts Practical Answers at Your Finger Tips Each volume is designed for maximum convenience with a concise, easy-to-follow format filled with visually-appealing features, including illustrations, graphs, diagrams, troubleshooting tables, and more. This approach offers all food professionals -- not just technical professionals -- quick access to the basic technical knowledge needed to understand and work with specific ingredients. Basic Concepts. Production, Storage, and Handling. Common Enzyme Reactions. Analysis of Enzyme Activity. Application of Enzymes to Baked Products. Enzyme Applications for Beverages. Other Applications. Choosing Enzymes for Specific Applications. Glossary. Index.
"Here is the full story of the discovery and history of these magical catalysts: how they work, how the body makes them, how the chemist studies them, and how they are produced commercially ... [Also included is information] on enzyme structure and function, and on the current theories of the part enzymes may have played in the origin of life on earth." Publisher's note.
"Microbial enzymes are important because they can be used for a wide variety of industrial purposes. There is dispersed and scanty information available with respect to microbial enzymes and their industrial applications. In this edited book, leading scientists have covered the various aspect of microbial enzymes and their industrial applications. Using microbial enzymes can help expedite various manufacturing processes and contribute to sustainable development, which is a priority worldwide. Research gaps in the entrainment of microbial enzymes with their direct application in product development are a major focus of this volume. Key Features: Cover microbial enzymes with comprehensive and in-depth information. Benefits students by describing recent advancements into microbial enzymology. Provides updates regarding microbial enzymes for researchers and industrial scientists. Includes findings on the microbial actions for better life Related Titles Thatoi, H., et al., eds. Microbial Fermentation and Enzyme Technology (ISBN 978-0-3671-8384-4). Svendsen, A., ed. Understanding Enzymes: Function, Design, Engineering, and Analysis (ISBN 978-9-8146-6932-0). Seneviratne, C. J., ed. Microbial Biofilms: Omics Biology, Antimicrobials and Clinical Applications (ISBN 978-0-3676-5799-4). Suzuki, H. How Enzymes Work: From Structure to Function (ISBN 978-9-8148-0066-2). de Lourdes, M., et al., eds. Fungal Enzymes (ISBN 978-1-4665-9454-8)"--
This edited work presents studies that clarify several aspects of the development and application of therapeutic enzymes. Therapeutic enzymes exhibit fascinating features and opportunities, and represent a significant and promising subcategory of modern biopharmaceuticals for the treatment of several severe diseases. Research and drug developments efforts and the advancements in biotechnology over the past twenty years have greatly assisted the introduction of efficient and safe enzyme-based therapies for a range of both rare and common disorders. The introduction and regulatory approval of twenty different recombinant enzymes has enabled effective enzyme-replacement therapy. This book covers mainly three areas of recombinant therapeutic enzymes and their clinical and pharmaceutical technology: (i) overview of the production process and biochemical characterization of therapeutic enzymes, (ii) focuses upon the engineering strategies and delivery methods of therapeutic enzymes, (iii) clinical applications of selected therapeutic enzymes, including aspects on their mechanisms of action and information on safety, immunogenicity issues and various adverse events of the enzymes used for therapy. The topic of this book is particularly relevant to academics, researchers and students undertaking advanced undergraduate/postgraduate programs in the biopharmaceutical/biotechnology area who wish to gain a comprehensive understanding of enzyme-based therapeutic molecules.
As an important class of catalysts that exhibit high efficiency and specificity in cellular environment, enzymes control various signaling cascades of biological systems. Self-assembly, a process that autonomously reorganizes components also plays important roles in maintaining the cell integrity and functions. Enzyme instructed self-assembly is a process that integrates enzymatic transformation and molecular self-assembly. That is, an enzyme catalyzes the conversion of a precursor to a molecule that self-assembles in aqueous solution, which usually results in hydrogelation. In this thesis, we discuss how enzymes trigger supramolecular self-assembly of small molecules to form hydrogels and the relevant applications. As a multi-step molecular process, enzyme mediated self-assembly is able to take place pericellularly, intracellularly, in vitro, or in vivo. Various enzymes, such as phosphatases, esterases, ?-lactamases, and thermolysins, can catalyze the process. Here I describe some of my works on enzyme instructed self-assembly, which show promising applications in tissue engineering and cancer therapy. The expression levels of enzymes may vary depending on the types of cells, tissues, or organs, so the utilization of enzymes can control the response of the self-assembly according to the biological environment. Thus, enzyme instructed self-assembly promises new applications in biomedicine since the integration of the inherent enzymatic reactions with self-assembly achieves excellent spatiotemporal control. Besides enzyme instructed self-assembly, the development of supramolecular chemistry has inspired researchers to explore more properties of self-assemblies. Chapter 5 describes the application of aromatic-aromatic interactions to reconsititute the original secondary structures of isolated peptides which originated from proteins. The simply conjugation of aromatic motifs to the isolated peptides can successfully restore their secondary structure which may lead to more applications. Chapter 7 talks about the work that we used supramolecular self-assemblies to explore how the stability of peptides in biological environment and the proteolysis of these peptides affect the activities of the peptide nanofibers against cancer cells. These works indicate that there can be more applications of supramolecular self-assemblies in chemical or biological fields.
Enzymes as chemical catalysts; Protein Structure; Nomenclature, Coenzymes and methods in enzymology; Gactors affecting enzyme activity; The molecular structure of enzymes.
Leading experts from all over the world present an overview of the use of enzymes in industry for: - the production of bulk products, such as glucose, or fructose - food processing and food analysis - laundry and automatic dishwashing detergents - the textile, pulp and paper and animal feed industries - clinical diagnosis and therapy - genetic engineering. The book also covers identification methods of new enzymes and the optimization of known ones, as well as the regulatory aspects for their use in industrial applications. Up to date and wide in scope, this is a chance for non-specialists to acquaint themselves with this rapidly growing field. '...The quality...is so great that there is no hesitation in recommending it as ideal reading for any student requiring an introduction to enzymes. ...Enzymes in Industry - should command a place in any library, industrial or academic, where it will be frequently used.' The Genetic Engineer and Biotechnologist
